Journal: Cancers

Article Title: Exploring MicroRNA and Exosome Involvement in Malignant Pleural Mesothelioma Drug Response

doi: 10.3390/cancers14194784

Figure Lengend Snippet: Dose response curves depicting an enhanced MPM cell sensitivity to drug treatment with ( A ) cisplatin, ( B ) gemcitabine, ( C ) YM155 and ( D ) PND-1186, following transfection with various miRNAs (1 nM). The FAK inhibitor-resistant VMC23 cell line was used for all experiments involving PND-1186 treatment. The chemotherapy drug and survivin small molecule inhibitor-resistant MSTO-211H cell line was used for all experiments involving cisplatin, gemcitabine and YM155 treatment. All dose responses are shown with respect to the miRNA control mimic. Error bars represent the mean ± SD, as determined from three experimental replicates per tested drug concentration.

Article Snippet: Five MPM cell lines (H2052, H2452, H28, H226 and MSTO) and the immortalised mesothelial cell line, MeT-5A, were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Transfection, Control, Concentration Assay

Journal: Cancers

Article Title: Exploring MicroRNA and Exosome Involvement in Malignant Pleural Mesothelioma Drug Response

doi: 10.3390/cancers14194784

Figure Lengend Snippet: IC 50 values and fold change increase in MPM cell sensitivity to chemotherapy/small molecule inhibitor drug treatment following miRNA transfection pre-treatment of MPM cells.

Article Snippet: Five MPM cell lines (H2052, H2452, H28, H226 and MSTO) and the immortalised mesothelial cell line, MeT-5A, were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Transfection, Control

Journal: Cancers

Article Title: Exploring MicroRNA and Exosome Involvement in Malignant Pleural Mesothelioma Drug Response

doi: 10.3390/cancers14194784

Figure Lengend Snippet: Graphs depicting ( A ) MPM cell response to increasing concentrations of YM155 treatment; ( B ) quantified levels of exosome production in conditioned medium obtained from MPM cell cultures, with and without exosome inhibitor (GW4869), determined using an exosome prep kit; and ( C ) MSTO-211H response to increasing concentrations of YM155 following pre-treatment of MSTO-211H cells with the GW4896 exosome inhibitor, with respect to MSTO-211H cells not pre-treated with GW4896. Error bars represent the mean ± SD, as determined from three experimental replicates. Statistically significant differences in exosome production between MPM cells treated with and without exosome inhibitor (GW4869) are indicated with a single asterisk (*) for a p -value of ≤0.05 and with a double asterisk (**) for a p -value of ≤0.01.

Article Snippet: Five MPM cell lines (H2052, H2452, H28, H226 and MSTO) and the immortalised mesothelial cell line, MeT-5A, were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: Cancers

Article Title: Exploring MicroRNA and Exosome Involvement in Malignant Pleural Mesothelioma Drug Response

doi: 10.3390/cancers14194784

Figure Lengend Snippet: Graphs depicting ( A ) mRNA expression levels corresponding to genes known to play a role in chemotherapy drug resistance, relative to the non-malignant MeT-5A control, as determined via qPCR analysis on a range of MPM cell lines; ( B ) mRNA levels corresponding to the ABCA6 and ABCA10 genes, relative to the non-malignant MeT-5A control, as determined via qPCR analysis on a range of MPM cell lines; MSTO-211H growth response to increasing concentrations of YM155 following silencing of the ( C ) ABCA6 and ( D ) ABCA10 genes. The dose response curves of the siRNA-treated cells are shown with respect to the untreated miRNA control mimic. Error bars represent the mean ± SD, as determined from three experimental replicates.

Article Snippet: Five MPM cell lines (H2052, H2452, H28, H226 and MSTO) and the immortalised mesothelial cell line, MeT-5A, were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Control

Journal: BMC Cancer

Article Title: Proteomics of malignant pleural mesothelioma under hypoxic and normoxic conditions in a large animal (porcine) tumor model

doi: 10.1186/s12885-025-15256-9

Figure Lengend Snippet: Malignant mesothelioma in Tibetan pig No. 3, epithelioid type. A Gross examination revealed the pleural tissue as a pale grayish-brown mass with an infiltrative tendency. B H&E staining showed the tumor tissue in a nodular pattern, with localized glandular and micropapillary formations, large tumor cells with eosinophilic or clear cytoplasm, prominent nucleoli, visible mitotic figures, some plasma cell-like tumor cells, and focal necrosis within the tumor tissue. C A few tumor cells expressed CK7 positively. D The Ki-67 proliferation index was approximately 10% positive. E A portion of tumor cells showed positive expression for wt-1. F Calretinin was diffusely positive in tumor cells. Vimentin was positively expressed in some tumor cells and interstitial fibroblasts, D2-40 showed weak positive expression in some tumor cells, a portion of tumor cells exhibited weak positive expression for MC, CD31 was positive in small vascular endothelial cells but not expressed in tumor cells, and NapsinA was negatively expressed in tumor cells. Scale bar: B-F 60μm

Article Snippet: Human pleural mesothelioma cells (MSTO-211 H) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. .

Techniques: Staining, Clinical Proteomics, Expressing

Journal: BMC Cancer

Article Title: Proteomics of malignant pleural mesothelioma under hypoxic and normoxic conditions in a large animal (porcine) tumor model

doi: 10.1186/s12885-025-15256-9

Figure Lengend Snippet: Malignant mesothelioma in Landrace pig No. 1, epithelioid type. A Gross examination showed the pleural tissue as a brownish-gray mass with minimal infiltration. B H&E staining revealed a small number of tumor cells arranged in nests and strands, predominantly epithelioid with eosinophilic cytoplasm, a few spindle cells, prominent nucleoli, and visible mitotic figures. C Vimentin was positively expressed in a few tumor cells and interstitial fibroblasts. D A portion of tumor cells expressed CK7 positively. E A portion of tumor cells showed positive expression for Calretinin. F The Ki-67 proliferation index was approximately 5-10% positive. A few tumor cells expressed D2-40 positively, a minority of tumor cells expressed MC positively, a portion of tumor cells exhibited weak positive expression for wt-1, CD31 was positive in small vascular endothelial cells but not expressed in tumor cells, and NapsinA was negatively expressed in tumor cells.Scale bar: B-F 60μm

Article Snippet: Human pleural mesothelioma cells (MSTO-211 H) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. .

Techniques: Staining, Expressing

Journal: BMC Cancer

Article Title: Proteomics of malignant pleural mesothelioma under hypoxic and normoxic conditions in a large animal (porcine) tumor model

doi: 10.1186/s12885-025-15256-9

Figure Lengend Snippet: Malignant mesothelioma in Landrace pig No. 2, epithelioid type. A Gross examination revealed the pleural tissue as a grayish-brown mass with infiltrated boundaries. B H&E staining showed tumor cells infiltrating the pleural stroma, arranged in diffuse sheets and small nests, with relatively uniform cell size and morphology, eosinophilic cytoplasm, rare mitotic figures, vascular proliferation within the stroma, and some inflammatory cell infiltration around the tumor cells. C A portion of tumor cells exhibited weak positive expression for CK7. D The Ki-67 proliferation index was approximately 10% positive. E A few tumor cells showed weak positive expression for MC. F: Vimentin was positively expressed in some tumor cells and interstitial fibroblasts. A few tumor cells showed weak positive expression for D2-40, CD31 was positive in small vascular endothelial cells but not expressed in tumor cells, a portion of tumor cells showed positive expression for wt-1, Calretinin was negatively expressed in tumor cells, and NapsinA was negatively expressed in tumor cells. Scale bar: B-F 60μm

Article Snippet: Human pleural mesothelioma cells (MSTO-211 H) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. .

Techniques: Staining, Expressing

Journal: BMC Cancer

Article Title: Proteomics of malignant pleural mesothelioma under hypoxic and normoxic conditions in a large animal (porcine) tumor model

doi: 10.1186/s12885-025-15256-9

Figure Lengend Snippet: Malignant mesothelioma in Tibetan pig No. 1, biphasic with approximately 75% epithelioid component. A Gross examination revealed the pleural tissue as a pale brownish mass with clearly infiltrated boundaries. B H&E staining showed the tumor tissue arranged in small nests and strands, predominantly composed of epithelioid cells with eosinophilic or basophilic cytoplasm, interspersed with a few spindle cells. The presence of large atypical cells, prominent nucleoli, and readily observable mitotic figures was noted. The stroma was vascular-rich, containing tumor emboli within the vessels, and scattered multinucleated giant tumor cells were observed at the tumor periphery. C CD31 was positive in small vascular endothelial cells but not expressed in tumor cells. D The majority of tumor cells expressed CK7 positively. E Vimentin was positively expressed in tumor cells and interstitial fibroblasts. F The Ki-67 proliferation index was approximately 70% positive. A minority of tumor cells expressed MC positively, a portion of tumor cells showed positive expression for Calretinin, and weak positive expression of wt-1 was observed in some tumor cells. D2-40 and NapsinA were negatively expressed in tumor cells. Scale bar: B-F 60μm

Article Snippet: Human pleural mesothelioma cells (MSTO-211 H) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. .

Techniques: Staining, Expressing

Journal: BMC Cancer

Article Title: Proteomics of malignant pleural mesothelioma under hypoxic and normoxic conditions in a large animal (porcine) tumor model

doi: 10.1186/s12885-025-15256-9

Figure Lengend Snippet: Malignant mesothelioma in Tibetan pig No. 2, biphasic with approximately 80% epithelioid component. A Gross examination showed the pleural tissue as a pale grayish-brown mass with infiltrated boundaries. B H&E staining revealed the tumor tissue arranged in nests and strands, predominantly composed of epithelioid cells with eosinophilic or basophilic cytoplasm, interspersed with a few spindle cells. The presence of large atypical cells, prominent nucleoli, and readily observable mitotic figures was noted. Surrounding the tumor, hemorrhage and abundant fibrinous exudates were present. C A portion of tumor cells exhibited weak positive expression for wt-1. D The Ki-67 proliferation index was approximately 60% positive. E The majority of tumor cells expressed CK7 positively. F A portion of tumor cells showed positive expression for Calretinin.Vimentin was positively expressed in a few tumor cells and interstitial fibroblasts, D2-40 was partially positive in tumor cells, CD31 was positive in small vascular endothelial cells but not expressed in tumor cells, a minority of tumor cells expressed MC positively, and NapsinA was negatively expressed in tumor cells. Scale bar: B-F 60μm

Article Snippet: Human pleural mesothelioma cells (MSTO-211 H) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. .

Techniques: Staining, Expressing